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Nucleic Acid Blotting
Contributor(s): Darling, D. C. (Author), Bricknell, P. M. (Author)

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 ISBN: 0199634467     ISBN-13: 9780199634460
Publisher: Garland Science
OUR PRICE: $37.00  

Binding Type: Paperback - See All Available Formats & Editions
Published: June 1994
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Annotation: Nucleic acid hybridization techniques allow the detection of specific DNA or RNA sequences. This book is a clear and concise guide to the techniques used for preparing DNA and RNA for membrane hybridization. These include Southern blotting of DNA, northern blotting of RNA, dot/slot blotting,
Benton-and-Davis screening of recombinant bacteriophage and Grunstein-Hogness screening of recombinant plasmids. It also discusses the pros and cons of using nitrocellulose filters and nylon membranes in these procedures. The book demystifies the laboratory manuals by explaining the rationale for
each step in the published protocols and points out potential pitfalls with tips on how to avoid them.
The ideal support for newcomers - provides the answers to all the questions you want to ask.

Click for more in this series: Basics
Additional Information
BISAC Categories:
- Science | Life Sciences - Cell Biology
- Medical
- Science | Life Sciences - Biology
Dewey: 574.873
LCCN: 94019264
Series: Basics
Physical Information: 0.26" H x 7.44" W x 9.69" L (0.52 lbs) 128 pages
Themes:
- Topical - Ecology
Features: Bibliography
 
Descriptions, Reviews, Etc.
Publisher Description:
Nucleic acid hybridization techniques allow the detection of specific DNA or RNA sequences. This book is a clear and concise guide to the techniques used for preparing DNA and RNA for membrane hybridization. These include Southern blotting of DNA, northern blotting of RNA, dot/slot blotting,
Benton-and-Davis screening of recombinant bacteriophage and Grunstein-Hogness screening of recombinant plasmids. It also discusses the pros and cons of using nitrocellulose filters and nylon membranes in these procedures. The book demystifies the laboratory manuals by explaining the rationale for
each step in the published protocols and points out potential pitfalls with tips on how to avoid them.

The ideal support for newcomers - provides the answers to all the questions you want to ask.
 
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